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http://inecol.repositorioinstitucional.mx/jspui/handle/1005/7| Identification, molecular characterization, and evolution of group I introns at the expansion segment D11 of 28S rDNA inRhizoctonia species | |
| MARIA DOLORES GONZALEZ HERNANDEZ | |
| Acceso Abierto | |
| Atribución-NoComercial-SinDerivadas | |
| Anamorph, LSU rDNA, Phytopathogenic fungi, RNA secondary structure, Thanatephorus | |
| The nuclear ribosomal DNA of Rhizoctonia species is polymorphic in terms of the nucleotide composition and length. Insertions of 349e410 nucleotides in length with characteristics of group I introns were detected at a single insertion point at the expansion segment D11 of 28S rDNA in 12 out of 64 isolates. Eleven corresponded to Rhizoctonia solani (teleomorph: Thanatephorous) and one (AG-Q) to Rhizoctonia spp. (teleomorph: Ceratobasidium). Sequence data showed that all but AG-Q contained conserved DNA catalytic core regions (P, Q, R, and S) essential for selfsplicing. The predicted secondary structure revealed that base- paired helices corresponded to subgroup IC1. Isolates from same anastomosis group and even subgroups within R. solani were variable with regard to possession of introns. Phylo- genetic analyses indicated that introns were vertically transmitted. Unfortunately, sequence data from the conserved region from all 64 isolates were not useful for delimiting species. Analyses with IC1 introns at same insertion point, of both Ascomycota and Basidio- mycota indicated the possibility of horizontal transfer at this site. The present study uncov- ered new questions on evolutionary pattern of change of these introns within Rhizoctonia species. | |
| 2013 | |
| Artículo | |
| Inglés | |
| González Hernández M.D. 2013. Identification, molecular characterization, and evolution of group I introns at the expansion segment D11 of 28S rDNA inRhizoctonia species. Fungal Biology. 117: 623-637 | |
| BIOLOGÍA MOLECULAR | |
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| 2433_2013-10420.pdf | Artículo publicado | 3.01 MB | Adobe PDF | Visualizar/Abrir |